School of Medicine

Wayne State University School of Medicine
K.V. Honn Cancer & Metastasis Research Group
Department of Pathology

Profile

Menq-Jer Lee, PhD


Associate Professor
(313) 577-1018
eb8578@wayne.edu
431 Chemistry Bldg., Detroit, Michigan 48202

Curriculum Vitae

 

Curriculum Vitae
 
 
NAME                                      :           Menq-Jer Lee
 
CITIZENSHIP                          :           USA
MARITAL STATUS                  :           Married with two children
ADDRESS                  (Lab.)   :           Department of Pathology,
                                                            Wayne State University School of Medicine                          
TELEPHONE (Lab.)               :           (313) 577-1041
E-mail                                      :           menqjer.lee@wayne.edu
                                                                       
Education:
Oct, 1996 – May 1999 :           Senior Research Associate,
                                                            Department of Physiology/ U. of Connecticut Health Center
Nov, 1993 - Sep, 1996            :           Postdoctoral Fellow/ Department of Molecular Biology
                                                            Jerome H. Holland Laboratory/ American Red Cross
Oct, 1987 - Oct, 1993             :           Ph.D. Candidate/ Dept. of Anatomy and Cell Biology,
                                                            McGill University, Montreal, Quebec, Canada.
July, 1985 - June, 1987           :           Second Lieutenant (obligatory army service)/ Taiwan Army Force
Sep, 1982 - Feb, 1985            :           M. Sc/ Dept. of Microbiology and Immunology,
                                                            National Taiwan Yang-Ming University.
Sep, 1978 - May, 1982            :           B.Sc./ National Taiwan Ocean University.
 
A) Position/ Employment:
Sep. 1999 – Sep. 2003           :           Assistant Professor/ Center for Vascular Biology
                                                            Department of Physiology/ U. of Connecticut Health Center
Sep. 2002 – Aug. 2003           :           Assistant Director/ Proteomics Center/ U. of Conn. Health Center
Sep. 2003 – Aug 2009            :           Assistant Professor/ Department of Microbiology and Immunology,
University of Louisville School of Medicine
Sep. 2009 – Present               :           Associate Professor/ Department of Pathology
                                                            Wayne State University School of Medicine
 
B) MEMBERSHIPS
American Society of Cell Biology
 
C) AWARDS AND HONORS
1.         B.Sc. in Dean's honor list (1982)
2.         Research studentship, Taiwan Yang-Ming Medical University (1982 - 1985)
3.         Research studentship, McGill University, Montreal, Canada (1987 - 1993)
4.         Ph.D. in McGill Dean's honor list (1993)
5.         Research Initiation Support and Enhancement Award. University of Connecticut
Health Center. (May/ 2000 - April/ 2001).
6.         Scientist Development Award. American Heart Association/ Heritage Affiliate.
            (July/ 2000 - June/ 2003).
7.         Travel Award (2003) Proteomics in Diabetes Conference, NIH
 
D)        TEACHING
1.         Graduate Cell Biology(2003-2009): three lectures: (a) cell/cell junctions, extracellular matrix, (b) Cell/matrix interactions, adhesion molecules and cell trafficking, and (c) Proteomics.
2.         Research Methods in Microbiology and Immunology (2003-2009): two lectures: (a) High through-put proteomics: a combination of automatic HPLC and LC-MS/MS, (b) Viral-mediated             siRNA gene silencing and miRNA mediated gene regulation.
 
E)        POSTDOCTOR TRAINING:
1.         1999 - 2000:                Karim Mohammed, PhD.
2.         2000 - 2003:                Harunobu Ozaki, MD, PhD.
3.         2004 - 2004:                Nazrin Khoshkhoo, PhD.
4.         2004 - 2007:                Harshini Sarojini, PhD.
5.         2004 - 2007:                Yuanqi Zhu, PhD.
6.         2008 - 2009:                Rosendo Estrada, PhD.
7.         2009 - present:            Jingjing Liu, MD, PhD
8.         2009 - present:            Wenliang Zhang, PhD
 
F)         GRADUATE STUDENT TRAINING
1.         Lichun Wang (role: Supervisor. 2004-2007. Dr. Wang is currently a postdoc in U. of Chicago)
2.         Deanna Siow (role: Dissertation Committee, Dep. of Biochem & Mol. Biol., 2006-2009)
3.         Christelle El Haibi (role: Dissertation Committee, Dep. of Microbiol. & Immunol., 2007-2009)        
4.         Roy Joseph (Role: M.Sc. Thesis Examine Committee, Department of Biochemistry and  Molecular Biology)
           
G)        K-12 EDUCATION: 
1.         Ingle Chen (2006, Meyzeek middle School): Louisville Regional Science Fair (First place),            Kentucky State Science Fair (First place).
2.         Barclay Lee (2006, Meyzeek middle School): Louisville Regional Science Fair (Second place).
3.         Melissa Hou (Manual High School): Kentucky State Science Fair (First place, Medicine/    Science, 2005), Louisville Regional Science Fair (First Place, Medicine/Science, 2006).
4.         Vivien Chen (2006, Manual High School): Louisville Regional Science Fair (First Place,     Microbiology).    
5.         Jen-Fu Lee (2006, Manual High School): Louisville Regional Science Fair (First place,       Biochemistry), Kentucky JR. Academy of Science Research Paper Competition (Second             place, Biochemistry).
 
H)        SERVICE
1.         PHD candidate qualifying exam committee (2004-2008)
2.         Departmental Curriculum Committee (2007-2009)
 
I)          GRANT REVIEW
            Vascular Wall Biology Study Section, Region I, American Heart Association, 2008-2009.
            Ad hoc reviewer, Human Frontier Science Program
            Ad hoc reviewer, Austrian Science Foundation
            Ad hoc reviewer, NIA postdoctoral fellowship award
            Ad hoc reviewer, Wellcome Trust
            Ad hoc reviewer, Netherlands Organization for Health Research and Development.
 
J)         Editorial Board
            World Journal of Biological Chemistry
 
K)        MANUSCRIPT REVIEW  
            Nature Drug Discovery
            Journal of Biological Chemistry
            Science Signaling
            Cancer Research
            Trends in Endocrinology and Metabolism
            Cardiovascular Research
            Mechanism of Ageing and Development
            Scientific Word
 
L)         INVITED TALKS
 
1.         The joint meeting of McGill Center for Studies in Aging and the Bloomfield Center for Research    in Aging. May 5th, 1989. Montreal
"Young Quiescent and Senescent Cells can be Distinguished by the Persistent Expression of Statin in Response to Serum Addition"
 
2.         20th Annual Scientific and Education Meeting of The Canadian Association on Gerontology.          October 24 - 27, 1991. Toronto
"Identification of a 45 Kilodalton Serine / Threonine Kinase Associated with Statin, A non-Proliferative Cell specific Protein."
 
3.         The Joint Meeting of the American Society for Biochemistry and Molecular Biology, American       Society for Investigative Pathology, American Association of Immunologists, and ASBMB        Satellite Meetings. June 1 - 6, 1996. New Orleans,    Louisiana.
"Signal Transduction and Developmental Expression of Edg-1, an Inducible G-protein Coupled Receptor."
 
4.            The 73th Annual Meeting of The Japanese Biochemical Society. September 11 - 14, 2000. Yokohama, Japan.
"Sphingosine-1-phosphate: an extracellular-acting bioactive lipid that regulates angiogenesis."
 
5.         Cardiovascular Research Institute, University of South Dakota Health Center. April, 2003.
"Sphingosine-1-phosphate signaling: a novel modulator of endothelial activation and angiogenesis.
 
6.         " Proteomics in Diabetes. Bethesda, MD, NIH. 2003.
"Protein profiling of nuclear proteins regulated by EDG-1, a high affinity G-protein coupled receptor for sphingosine-1-phosphate."
 
7.         The Bumgardner Minisymposium on Microbial pathogenesis and immunity to infection.     Louisville, Kentucky, 2004.
“G-protein couple signaling in antimicrobial activity”
 
8.         45th Annual Meeting, American Society for Cell Biology, San Francisco, December 14, 2005.        ECIS (Electric Cell-substrate Impedance Sensing) Tutorial Workshop
“Role of ZO-1 in sphingosine-1-phosphate receptor regulated endothelial    barrier formation and chemotaxis”.
 
9.         Invited talk to MD/ PhD program, University of Louisville (March 6, 2007)
“Novel mechanism of angiogenesis mediated by serum-borne bioactive lipid”
 
10.       Molecular Target Seminar Series, University of Louisville (July 26, 2007)
“Sphingolipids Signaling in Vasculature”
 
11.       Allergan Inc., Irvin, California (May 5, 2008)
“Sphingosine-1-phosphate Signaling: A novel regulator of vascular permeability”
 
12.       Wayne State University, Detroit, Michigan (July 8, 2008)
“Sphingosine-1-phosphate signaling: the Yin-Yang regulation of vascular function, inflammation, and tumorigenesis”
 
13.       The 18th South East Lipid Research Conference (SELRC) (Sept. 24-27, 2009), Callaway Gardens, Pine Mountain, Georgia.
“Yin-Yang regulation of vascular functions by sphingosine-1-phosphate signaling”
 
14.       The 11th International Conference of Bioactive Lipid in Cancer, Inflammation, and Related Diseases (Cancun, Mexico, October 25 - 28, 2009)
"Balance of S1P1R and S1P2R signaling regulates vascular functions in vitro and in vivo.”
 
M)        POSTER PRESENTATION
 
1.         The Fourth International Congress of Cell Biology.
            14 - 19 August 1988, Montreal, Quebec, Canada.
2.         The Joint Meeting of The American Society for Cell Biology and the American
            Society for Biochemistry and Molecular Biology.
            29 January - 2 February 1989, San Francisco, California
3.         The Twenty - Ninth Annual Meeting of The American Society for Cell Biology
            5 - 9 November 1989, Houston, Texas.
4.         The Thirtieth Annual Meeting of The American Society for Cell Biology
            9 - 13 December 1990, San Diego, California.
5.         The Thirty - First Annual Meeting of The American Society for Cell Biology
            8 - 12 December 1991, Boston, Massachusetts.
6.         The Cell Cycle'93: Regulators, Targets and Clinical Applications.
            XIII Washington International Spring Symposium. 10 - 14 May 1993, Washington, D.C.
7.         The Thirty - fifth Annual Meeting of The American Society for Cell Biology
            9 - 13 December 1995, Washington, DC.
8.         Special Poster Session, The Thirty - seventh Annual Meeting of The American Society for Cell Biology. 13 - 17 December 1997, Washington, DC.
9.         G-Protein Signaling Workshop. Mount Sinai School of Medicine, 8 April, 1999, New York, NY.
10.       XI International Vascular Biology Meeting. 5 – 9 September 2000, Geneva, Switzerland.
11.       " Proteomics in Diabetes. Bethesda, MD, NIH. 2003.
12.       JF Lee, MJ Lee (2003). The Dual Physiological Functions of Lysophosphatidyl-choline, a Serum-borne Bioactive Lipid, on Macrophage Migration and Death. March 2003, Dupont Manual Science Fair, Louisville, Kentucky.
13.       L Wang, JF Lee, J Cai, H Sarojini, Q Zeng, C H. Liu, E Wang, T Hla, C Y Lin, MJ Lee (2005).       Role of avb3 integrin in Sphingosine-1-phosphate receptor regulated pro-angiogenic activity.        October 31-November 4, 2005. Research!Louisville 2005. Louisville, Kentucky.
14.       JF Lee, H Ozaki, T Hla, E Wang, MJ Lee (2005). Role of ZO-1 in Sphingosine-1-phosphate         receptor regulated endothelial barrier formation and chemotaxis. December 10-14, 2005, The         45th annual meeting of the American Society for Cell Biology, San Francisco, California.
15.       L Wang, JF Lee, J Cai, H Sarojini, Q Zeng, CH. Liu, E Wang, T Hla, CY Lin, MJ Lee (2005).         Role of avb3 integrin in Sphingosine-1-phosphate receptor regulated pro-angiogenic activity.        December 10-14, 2005, The 45th annual meeting of the American Society for Cell Biology, San        Francisco, California.
16.       R Estrada, JF Lee, B Haribabu, MJ Lee (2008). Up-regulating S1P2 Signaling Impairs Chemotactic, Wound-healing, and Morphogenetic Responses in Senescent Endothelial Cells. September 11-14, Hyannis, MA, North American Vascular Biology Organization.
17.       JF Lee,D Lominadze, and MJ Lee (2008)Balance of S1P1 and S1P2 Signaling Regulates Peripheral Microvascular Permeability in Rat Cremaster Muscle Vasculature. September 11-14, Hyannis, MA, North American Vascular Biology Organization.
18.       JF Lee and MJ Lee (2009) Sphingosine-1-phsophate Signaling Regulates Vascular Permeability. September 25-27, Pine Mountains, Georgia. South East Lipid Research Conference.
 
N)        PUBLICATIONS
 
1.         MJ Lee and E. Wang (1988) Persistent Expression of Statin is a Marker for Distinguishing Reversible from Irreversible Growth Arrest. J. Cell Biol. 107: 79a.
2.         T. Nakamura, MJ Lee, and E. Wang (1989) Expression of Statin, A Non-proliferating Cell-Specific Protein, Is Transcriptionally Controlled in Growth-Arrested State. J. Cell Biol. 109: 147a.
3.         R Bissonnette, MJ Lee, and E Wang. (1990) The differentiation process of intestinal epithelial cells is associated with the appearance of statin, a non-proliferation specific nuclear protein. J. Cell Sci. 95: 247-254.
4.         D. K. Ann, I. K. Moutsatsos, T. Nakamura, H. H. Lin, MJ Lee, S. Chin, R, Liem, E. Wang (1990) Isolation and Characterization of a Rat Chromosomal Gene for a Polypeptide Antigenically Related to Statin. J. Cell Biol. 111: 497a.
5.         DK Ann, IK Moutsatsos, T Nakamura, HH Lin, PL Mao, MJ Lee, S Chin, RKH Liem, and E Wang. (1991) Isolation and characterization of the rat chromosomal gene for a polypeptide (pS1) antigenically related to statin. J. Biol. Chem. 266: 10429-10437.
6.         MJ Lee, M. Sandig, and E. Wang (1991) Statin, A Nuclear Protein Specific for Non-proliferating Cells, Is A Phosphoprotein and Forms An In Vivo Complex with A p45 Serine / Threonine Kinase. J. Cell Biol. 115: 18a.
7.         HM Schipper, R Mauricette, JJ Liang, MJ Lee and E Wang. (1992) Expression of the non-proliferation-specific protein, statin, in grey matter neuroglia of the aging rat brain. Brain Res. 591: 129-136.
8.         MJ Lee, M Sandig, and E Wang. (1992) Statin, a protein specifically present in non-proliferating cells, is a phosphoprotein and forms a complex with a 45 kilodalton serine / threonine kinase. J. Biol. Chem. 267: 21773-21781.
9.         E Wang, MJ Lee, and S Pandey (1993) Control of fibroblast senescence and activation of programmed cell death. J. Cell Biochem. 54: 1-8.
10.       E Wang and MJ Lee (1994). Cell Cycle traverse and growth control in senescent human fibroblasts. The Cell Cycle: Regulators, Targets and Clinical Applications. Edited by Valerie Hu. Plenum Publishing, pp. 67-78.
11.       M. Evans, MJ Lee, and T. Hla (1995) Direct Interaction of the i3 Domain of the G-protein Coupled Receptor EDG-1 and Gi2 Polypeptide. Mol. Biol. Cell 6: 128a.
12.       MJ Lee, M. Evans, and T. Hla (1995) Interaction and Signalling of the Inducible G-protein Coupled Receptor EDG-1 with the Gi Pathway. Mol. Biol. Cell 6: 128a.
13.       MJ Lee, M Evans, and T Hla (1996) The inducible G protein-coupled receptor Edg-1 signals via the Gi/mitogen-activated protein kinase pathway. J. Biol. Chem. 271: 11272-11279.
14.       MJ Lee, C. H. Liu, and T. Hla (1996) Signal Transduction and Development Expression of Edg-1, an Inducible G-protein Coupled Receptor. The FASEB Journal 10: A1002.
15.       T Hla, A Ristimaki, K Narko, P Ben-Av, MJ Lee, M Evans, CH Liu, and H Sano (1996). Angiogenesis: Molecular Biology and Clinical Aspects. Edited by Macagondakis, M.E. Plenum Press, NY. Pp. 191-198.
16.       MJ Lee, JR Van Brocklyn, S Thangada, C Liu, AR Hand, R Menzeleev, S Spiegel and T Hla (1998) Sphingosine-1-phosphate as a ligand for the G protein-coupled receptor EDG-1. Science 279: 1552-1555.
17.       MJ Lee, S Thangada, C Liu, BD Thompson and T Hla (1998) Lysophosphatidic acid stimulates the G-protein-coupled receptor EDG-1 as a low affinity agonist. J. Biol. Chem. 273: 22105-22112.
18.       JR VanBrocklyn, MJ Lee, R Menzeleev, A Olivera, L Edsall, O Cuvillier, DM Thomas, S Thangada, T Hla, and S Spiegel (1998) Dual actions of sphingosine-1-phosphate: extracellular through the Gi-coupled orphan receptor EDG-1 and intracellular to regulate proliferation and survival. J. Cell Biol. 142: 229-240.
19.       T Hla, MJ Lee, N Ancellin, CH Liu, S Thangada, BD Thompson, and M Kluk (1999) Sphingosine-1-phosphate: extracellular mediator or intracellular second messenger? Biochem. Pharmacology. 58: 201-207
20.       CH Liu, S Thangada, MJ Lee, JR Van Brocklyn, S Spiegel, and T Hla (1999) Ligand induced trafficking of the sphingosine-1-phosphate receptor EDG-1. Mol. Biol. Cell. 10: 1179-1190
21.       RT Windh, MJ Lee, T Hla, S An, AJ Barr, and DR Manning (1999) Differential coupling of the sphingosine-1-phosphate receptors Edg-1, Edg-3, and H218/Edg-5 to the Gz, Gq, and G12 families of heterotrimeric G proteins. J. Biol. Chem. 274: 27351-27357.
22.       MJ Lee, S Thangada, KP Claffey, N Ancellin, CH Liu, M Kluk, M Volpi, RI Sha’afi and T Hla (1999). Vascular endothelial cell adherens junction assembly and morphogenesis induced by sphingosine-1-phosphate. Cell99: 301-312.
23.       T Hla, MJ Lee, N Ancellin, S Thangada, CH Liu, M Kluk, S-S Chae, M-T Wu. (2000) Sphingosine 1-phosphate signaling via the EDG-1 family of G-protein-couple receptors. Ann. N.Y. Acad. Sci. 905: 16 – 24.
24.       MJ Lee, S Thangada, CH Liu, B Thompson, N Ancellin, M Kluk, and T Hla. (2000) EDG-1: An Endothelial-derived G-protein-coupled Receptor for Bioactive Lipids Regulates Morphogenetic Differentiation. In: Gabor Rubanyi, ed. Angiogenesis in Health and Disease: Basic Mechanisms and Clinical Applications. New York: Marcel Dekker, Inc. 125 – 131.
25.       MJ Lee, S Thangada, K Claffey, N Ancellin, C Liu, T Hla (2000) Vascular endothelial cell adherens junction assembly and morphogenesis induced by sphingosine-1-phosphate. J. Submicroscopic cytol Pathol. 32: B148.
26.       Y Liu, R Wada, R Yamashita, Y Mi, CX. Deng, JP Hobson, HM Rosenfeldt, VE Nava, SS Chae, MJ Lee,CHLiu, T Hla, S Spiegel, and RL Proia. (2001) Edg-1, the G-protein coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation. J. Clin. Invest. 106: 951-961.
27.       JH Paik, SS Chae, MJ Lee, S Thangada, and T Hla. (2001). Sphigosine1-phosphate induced endothelial cell migration requires the expression of EDG-1 and EDG-3 receptors and Rho-dependent activation of avb3 and b1-containing integrin. J. Biol. Chem. 276: 11830-11837.   
28.       M Morales-Ruiz, MJ Lee, S Zöllner, JP Gratton, R Scotland, I Shiojima, K Walsh, T Hla, and WC Sessa. (2001) Sphingosine-1-phosphate activates Akt, nitric oxide production and chemotaxis through a Gi -protein/ phosphoinositide 3-kinase pathway in endothelial cells. J. Biol. Chem. 276:19672-19677.
29.       MJ Lee, S Thangada, JH Paik, GP Sapkota, N Ancellin, M Wu, M Morales-Ruiz, WC Sessa, D Alessi, T Hla (2001) AKT mediated phosphorylation of the G-protein-coupled receptor EDG-1 is required for endothelial cell chemotaxis. Molecular Cell 8: 1- 20.
30.       T Hla, MJ Lee, N Ancellin, JH Paik, MJ Kluk (2001) Lysophospholipid mediators. Science 294: 1875-1878.
31.       H Ozaki, T Hla, MJ Lee (2003) Sphingosine-1-phosphate signaling in endothelial activation. J. Atherosclerosis and Thrombosis 10: 125-132.
32.       JF Lee, H Ozaki, X Zhan, E Wang, T Hla, MJ Lee (2006) Sphingosine-1-phosphate signaling regulates lamellipodia localization of cortactin complexes in endothelial cells. Histochem Cell Biol. 14:1-8.
33.       JF Lee, Q Zeng, H Ozaki, L Wang, AR Hand, T Hla, E Wang, MJ Lee (2006) Dual roles of tight junction associated protein, Zonula Occludens-1, in sphingosine-1-phosphate regulated endothelial barrier integrity and wound-healing response. J. Biol. Chem. 281:29190-29200.
34.       MA Eskan, BG Rose, MR Benakanakere, MJ Lee, DF Kinane (2008)Sphingosine 1-phosphate 1 and TLR4 mediate IFN-b expression in human gingival epithelial cells. J. Immunol. 180: 1818-1825.
35.       L Wang, JF Lee, CY Lin, MJ Lee (2008) Rho GTPases mediated integrin avb3 activation in sphingosine-1-phosphate stimulated chemotaxis of endothelial cells. Histochem Cell Biol. 129: 579-588.
36.       H Sarojini,R Estrada, H Lu, S Dekova, M.J. Lee, RD. Gray, E Wang (2008) Pigment epithelium-derived factor identified in the secretome of a mouse mesenchymal stem cell is a major fibroblast chemoattractant.  J Cell Biochem. 104:1793-1802
37.       MA Eskan, BG Rose, M Benakanakere, Q Zeng, D Fujioka, M Martin, M.J. Lee, DF Kinane (2008) S1Preceptors act with TLR4 to enhance Inflammatory cytokines in human gingival epithelial Cells. Eur J. Immunol. 4:1138-1147.
38.       Estrada R, Zeng Q, Lu H, Sarojini H, Lee JF, Mathis SP, Sanchez T, Wang E, Kontos CD, Lin CY, Hla T, Haribabu B, MJ Lee (2008) Up-regulating sphingosine-1-phosphate receptor-2 signaling impairs chemotactic, wound-healing, and morphogenetic responses in senescent endothelial cells. J Biol Chem. 283: 30363-30375
39.       Estrada R, Wang L, Jala VR, Lee JF, Lin CY, Gray RD, Haribabu B, MJ Lee. (2009). Ligand-induced nuclear translocation of S1P(1) receptors mediates Cyr61 and CTGF transcription in endothelial cells. Histochem Cell Biol. 131: 239-251.
40.       Lee JF, Gordon S, Estrada R, Wang L, Siow DL, Wattenberg BW, Lominadze D, and MJ Lee (2009)Balance of S1P1 and S1P2 signaling regulates peripheral microvascular permeability in rat cremaster muscle vasculature. Am J Physiol Heart Circ Physiol. 296: H33-H42
41.       R Estrada, N Li, H Sarojini, MJ Lee, E Wang (2009) Murine mesenchymal stromal cells induces angiogenesis via cyr61 secretion. J Cell Physiol. 219:563-571.
42.       Dean WL, Lee MJ, Cummins TD, Schultz DJ, Powell DW (2009) Proteomic and functional characterisation of platelet microparticle size classes. Thromb Haemost. 102:711-718.
 
O) Research Supports
 
Completed
1.         Research Initiation Support and Enhance Award.                              7/2000 - 12/2001        
            University of Connecticut Health Center (PI)
 
Title: Akt/ PKB Functions in SPP/ EDG-1 Regulated Angiogenesis.
The goal of this support is to identify the novel p60 kinase in EDG-1 signaling.
Total award: 50,000.
 
2.            Scientist Development Award.                                                           7/2000 - 6/2003
            American Heart Association/ Heritage Affiliate (PI)
 
Title: Novel Signaling Molecules Regulated by the EDG-1 Receptor in Angiogenesis.
The major goal of this project is to characterize Akt/ PKB function in EDG-1 signaling.
Total direct cost: 180,000.
 
3.         Army Research Office/ Department of Defense (W911NF-05-1-0136)        04/01/2005
            (5% effort, Co-PI.)
 
Title:    High throughput Proteomic profiling of host-resistance to biowarfare pathogen’s infection.
Total $307,612 (used for purchasing Qstar XL Hybrid LC/ MS/ MS  Mass Spectrometer, Applied Biosystems).
 
4.         DARPA BAA04-12 (Co-PI, 30% effort)                                               05/2006 – 09/2008
            DARPA
 
Title: Digit Regeneration in Mammals
The milestone is to characterize the blastema in regeneration process
Total direct/indirect cost: $2,000,000.
 
Ongoing
1.         NIH 1 R01 HL71071                                                                            7/ 2003 - 6/ 2013
            NHLBI/ NIH (PI)
 
Title: Sphingolipid Signaling in Endothelial Function.
The goal of this project is to study signaling and functions of S1P1 in vascular endothelial cells.
Total direct cost: 250,000/ year.
 
2.         American Heart Association Grant-in-aid                                           07/2007 – 06/2009
            Great River Affiliate
Title: Nuclear Sphingosine-1-phosphate Receptor Subtype 1 in Endothelial Function.
The major goal of this project is to study the signaling and functions of nuclear S1P1 receptor.
Total direct cost: 121,000.
 
3.         NSF KyEPSCoR (Co-PI, 5% effort)                                                   10/2008-09/2013
                       
Title:    Engineering Platforms for Exploring Cellular and Molecular Signaling Processes.
This proposal, requesting a ~10 million budget from NSF/ KyEPSCoR, is to create the infrastructure for uniting a multidisciplinary, multiinstitutional team of faculty to focus on interfacing state-of-the-art engineering technology with biological and medical systems. Two biologists are involved in this application. I am responsible for study of vascular biology, Dr. Marnie Saunders (U of Kentucky) is responsible for study of bone morphogenesis.