Assistant Professor
(313) 577-2088
maddipati@wayne.edu
431 Chemistry Bldg.,Detroit, Michigan 48202

Fax

(313) 577-0798

Education

B.Sc., First Class, Andhra University, India, 1975
    Major: Chemistry,  Minor: Biology

 M.Sc., First Class,  Kurukshetra University, India, 1977
    Specialization:  Organic Chemistry

 Ph.D., Indian Institute of Science, India, 1983
    Dissertation:  "Microbial degradation of sterol side chains"
   

Research Interest

Biochemistry and chemistry of lipoxygenases, cyclooxygenases, and peroxidases, PUFA metabolism, Enzyme kinetics, and organic synthesis. Specifically, 12-lipoxygenase mediated gene regulation and 12(S)-HETE signaling mechanisms

Curriculum Vitae

Training:
Jan. 1983 - Aug. 1983    : Research Associate, Dept. of Science and Technol.,
     Indian Institute of Science, Bangalore, India
Aug. 1983 - Aug. 1985    : Research Associate, Ctr. for Air Environ. Studies,
     The Pennsylvania State University, University Park,  PA 16802
Aug. 1985  - Dec. 1987    : Research Associate, Department of Chemistry,
     Wayne State University, Detroit,  MI 48202

Professional Appointments:
Jan. 1988  -  July 1989    : Research Scientist, Department of Chemistry,
     Wayne State University, Detroit, MI 48202
Aug. 1989  - March 2001    : Director, Research and Development
     Cayman Chemical Company, Ann Arbor, MI 48108
Oct. 2001 – present    : Assistant Professor (Research), Radiation Oncology
     Wayne State University, Detroit, MI 48202

Major Professional Societies:
    Member of the American Chemical Society
    Member of the American Association for Cancer Research


Honors/Awards:
    Research Fellow, Council of Scientific and Industrial Research, India.  Feb. 1978-Dec. 1982.

Grant Support:
Current:
•    Purification and crystallization of human 12-LOX enzymes. NIH, P.I.: K. R. Maddipati, Period: 09/01/2004 - 08/30/2006, $101,295.
•    Anticancer Prodrug Development Program. Michigan Technology Tri-Corridor (MTTC), P.I.: K. R. Maddipati, Period: 10/01/05 - 09/30/08, $569,977.

Past:
•    Structural Characterization of Human Plasma Glutathione Peroxidase. WSU Molecular Biology Center, P. I. K. R. Maddipati, 8/1/1987 to 7/31/1988, $19,385.
•    Enzyme Immunoassay for 8-iso-2,3-dinor prostaglandin F1α – Development of Specific Antibodies. NIEHS, P. I. K. R. Maddipati, 5/15/1998 to 11/14/1998, $99,871.

Publications:

•    Original observations in refereed journals:
•    Kandouz, M., Nie, D., Pidgeon, G. P., Krishnamoorthy, S., Maddipati, K. R., and Honn, K. V.  Platelet-type 12-lipoxygenase activates NF-κB in prostate cancer cells. Prostaglandins and other Lipid Mediators (2003) 70, 189-204 (2003)
•    Johnson, J. and Maddipati, K. R.  Paradoxical effects of resveratrol on the two prostaglandin H synthases. Prostaglandins and other Lipid Mediators 56, 131-143 (1998)
•    Samokyszyn, V. M., Chen, T., Maddipati, K. R., Franz, T. J. Lehman, P. A., and Lloyd, R. V.  Free radical oxidation of (E)-retenoic acid by prostaglandin H synthase.  Chem. Res. Toxicol. 8, 807-815 (1995)
•    Johnson, J., Wimsatt, J. W., Buckel, S. D., Dyer, R. D., and Maddipati, K. R.  Purification and characterization of inducible prostaglandin H synthase from sheep placental trophoblasts.  Arch. Biochem. Biophys. 324, 26-34 (1995)
•    Samokyszyn, V. M., Freeman, J. P., Maddipati, K. R., and Lloyd, R. V.  Peroxidase-catalyzed oxidation of pentachlorophenol.  Chem. Res. Toxicol. 8, 349-355 (1995)
•    Brooks, B., Byers, L. W., Muirhead, E. E., Muirhead, M., Pitcock, J. A., Maddipati, K. R., and Maxey, K. M.  Purification of class I medullipins from the venous effluent of isolated normal kidneys perfused under high pressure with saline.  Blood Pressure 3, 407-417 (1994)
•    Odenwaller, R., Maddipati, K. R., and Marnett, L. J.  Detection of a higher oxidation state of manganese-prostaglandin endoperoxide synthase.  J. Biol. Chem. 267, 13863-13869 (1992)
•    Maxey, K. M., Maddipati, K. R., and Birkmeier, J.  Interference in enzyme immunoassays.  J. Clin. Immunoassay 15, 116-120 (1992).
•    O'Gara, C. Y., Maddipati, K. R., and Marnett, L. J.  Determination of hydroperoxide in human plasma by high pressure liquid chromatography using a coulometric electrochemical detector.  Chem. Res. Toxicol.  2,  (1989)
•    Marnett, L. J., Chen, Y.-N. P., Maddipati, K. R., Labeque, R., and Ple, P.  Localization of the peroxidase active site of PGH synthase.  Adv. Prostaglandin Thromboxane Leukotriene Res. 19, 458-461, (1989)
•    Reddy, C. C., Whelan, J., Maddipati, K. R., and Reddanna, P.  Mechanism of formation of leukotrienes and lipoxins from arachidonic acid catalyzed by homogeneous lipoxygenase from potato tubers.  Adv. Prostaglandin Thromboxane Leukotriene Res. 19, 132-136, (1989)
•    Marnett, L. J., Pan-Chen, Y. N., Maddipati, K. R., Ple, P., and Labeque, R.  Functional differentiation of cyclooxygenase and peroxidase activities of PGH synthase by trypsin treatment:  Localization of a probable prosthetic heme binding site.  J. Biol. Chem.  263, 16532-16535 (1988)
•    Whelan, J., Reddanna, P., Prasad, G., Maddipati, K. R., and Reddy, C. C.  Non-regiospecificity of the purified lipoxygenases from potato tubers.  Ann. N. Y. Acad. Sci. 524 393-394 (1988)
•    Maddipati, K. R., and Marnett, L. J.  Characterization of the major hydroperoxide-reducing activity of human plasma:  Purification and properties of a selenium-dependent glutathione peroxidase.  J. Biol. Chem.  262, 17398-17403 (1987)
•    Maddipati, K. R., Gasparsky, C., and Marnett, L.J.  Characterization of the hydroperoxide-reducing activity of human plasma.   Arch. Biochem. Biophys. 254, 9-17 (1987)
•    Chang, M., Maddipati, K. R., Reddanna, P., Li, C. H., Tu, C. P. D., Reddy, C. C., and Corey, E. J.  Specificity of the glutathione-S-transferase in the conversion of leukotriene A4 to leukotriene C4.  Arch. Biochem. Biophys. 259, 536-547 (1987)
•    Burgess, J. R., Yang, H., Chang, M., Maddipati, K. R., Tu, C. P-D., and Reddy, C. C.  Enzymatic transformation of PGH2 to PGF2α catalyzed by glutathione-S-transferases.  Biochem. Biophys. Res. Commun. 142, 441-447 (1987)
•    Li, C.-H., Srikumar, K., Burgess, J. R., Hong, Y., Hildenbrandt, G., Maddipati, K. R., Reddanna, P., Li, N., Tu, C. P. D., and Reddy, C. C.  Sheep lung glutathione S-transferases and eicosanoid biosynthesis.  in Contemporary Themes in Biochemistry (Eds. Kon, O. L. et al.) Vol. 6, pp. 662-663, Cambridge University Press, Cambridge, UK (1987)
•    Reddanna, P., Maddipati, K. R., and Reddy, C. C.  Inhibition of 5-lipoxygenase by vitamin E.  FEBS Letters  193, 39-43 (1985)
•    Maddipati, K. R., Madyastha, K. M., and Bhattacharyya, P. K.  Selective sterol side chain cleavage by Moraxella  Sp.  Proc. of VII th Int. Biotechnol. Symposium  330-331 (1984)
•    Bhattacharyya, P. K., Maddipati, K. R., Ramadevi, N., Malathi, R., Prema, M., and Madyastha, K. M.  Microbial oxidation of sterol side chains.  Ind. J. Chem. Soc. 56, 1-15 (1984)
•    Reddy, C. C., Maddipati, K. R., Mastro, A. M., and Egan, R. W.  Measurement of glutathione requiring enzymes involved in arachidonic acid cascade of Rat Basophilic Leukemic cells.  Biochem. Int. 9, 755-761 (1984)
•    Maddipati, K. R., Madyastha, K. M., and Bhattacharyya, P. K.  Novel transformations of cholesterol and 6β-methoxy i -cholesterol by Moraxella Sp.  J. Steroid Biochem. 19, 1391-1395 (1983)
•    Rao, G. V., Srinivasan, G. K., Murthy, N. V. S. R., and Maddipati, K. R.  Desilication efficiency versus sulfide loss in the carbonation of green liquor.  J. of  Ind. Pulp and Paper Tech. Assoc. 15, 111-115 (1978)


Review Articles:
Marnett, L. J. and Maddipati, K. R.  Prostaglandin H synthase  in Peroxidases in Biology and Chemistry, (eds. Everse, J., Everse, K.E., and Grisham, M.B.) Vol. 1, Chapter 13, pp. 293-334 (1991).

Published Abstracts:
Mechanisms Regulating Tumor Angiogenesis in Prostate Cancer by the Enzyme 12-Lipoxygenase in the Normoxic and Hypoxic Environment. S.Krishnamoorthy, D. Nie, R. Jin, K.R. Maddipati, K. Tang, and K. V. Honn. Prostaglandins and Other Lipid Mediators 79, 180-181 (2006).

Workshops/Courses/Invited Lectures:

•    ‘Interactions between Cyclooxygenase and Glutathione Peroxidases’ at International Conference on Biological Oxidations, Bangalore, India, 10/8/1989.
•    Workshop on Eicosanoids conducted at School of Life Sciences, University of Hyderabad, India, 12/4 – 12/7/1993.
•    ‘Biosynthesis and Metabolism of Eicosanoids’ at School of Life Sciences, University of Hyderabad, India, 12/3/1993.
•    ‘Spectroscopic Analysis of Organic Compounds’ Course taught at Cayman Chemical Company, Ann Arbor, MI, 8/1 – 12/20/1999.